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flow cytometry staining buffer  (Thermo Fisher)


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    Thermo Fisher flow cytometry staining buffer
    Flow Cytometry Staining Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry staining buffer/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    flow cytometry staining buffer - by Bioz Stars, 2026-04
    96/100 stars

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    (A) Experimental timeline of T-FUS+αCD40 dosing and harvest for flow <t>cytometry</t> analysis. (B) Average tumor outgrowth of E0771 tumor-bearing mice. Significance assessed by 2-way ANOVA, followed by Holm-Sidak multiple comparison correction. E0771: p<0.05 vs all other groups (specifically, IgG (n=5) vs T-FUS+αCD40 (n=12): p = <0.0001; T-FUS (n=7) vs T-FUS+αCD40: p =0.0843; αCD40 (n=7) vs T-FUS+αCD40: p = 0.0517). (C) Focused view of tumor outgrowth at selected timepoints (day 42-53) highlighting differences between T-FUS+αCD40 non-CRs and CRs, versus αCD40 monotherapy. (D) Waterfall plot depicting CRs in the combination treatment group. (E-H) Absolute number per gram tumor and percentage of CD8 + and CD4 + T cells in the tumor and (I-L) spleen. M) tSNE plot on CD45 + cells in the TDLN. (N-Q) Absolute number and percentage of CD8 + and CD4 + T cells in the TDLN. (R) Ratio between CD8 + and CD4 + T cells in the TDLN. Significance assessed using a Welch ANOVA or Welch’s T test. *p < 0.05, **p < 0.01 vs. T-FUS+αCD40 CRs.
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    (A) Experimental timeline of T-FUS+αCD40 dosing and harvest for flow cytometry analysis. (B) Average tumor outgrowth of E0771 tumor-bearing mice. Significance assessed by 2-way ANOVA, followed by Holm-Sidak multiple comparison correction. E0771: p<0.05 vs all other groups (specifically, IgG (n=5) vs T-FUS+αCD40 (n=12): p = <0.0001; T-FUS (n=7) vs T-FUS+αCD40: p =0.0843; αCD40 (n=7) vs T-FUS+αCD40: p = 0.0517). (C) Focused view of tumor outgrowth at selected timepoints (day 42-53) highlighting differences between T-FUS+αCD40 non-CRs and CRs, versus αCD40 monotherapy. (D) Waterfall plot depicting CRs in the combination treatment group. (E-H) Absolute number per gram tumor and percentage of CD8 + and CD4 + T cells in the tumor and (I-L) spleen. M) tSNE plot on CD45 + cells in the TDLN. (N-Q) Absolute number and percentage of CD8 + and CD4 + T cells in the TDLN. (R) Ratio between CD8 + and CD4 + T cells in the TDLN. Significance assessed using a Welch ANOVA or Welch’s T test. *p < 0.05, **p < 0.01 vs. T-FUS+αCD40 CRs.

    Journal: bioRxiv

    Article Title: Focused Ultrasound Thermal Ablation and CD40 Agonism Reprograms Breast Tumor Immunity to Drive Regression and Memory

    doi: 10.64898/2026.03.02.708396

    Figure Lengend Snippet: (A) Experimental timeline of T-FUS+αCD40 dosing and harvest for flow cytometry analysis. (B) Average tumor outgrowth of E0771 tumor-bearing mice. Significance assessed by 2-way ANOVA, followed by Holm-Sidak multiple comparison correction. E0771: p<0.05 vs all other groups (specifically, IgG (n=5) vs T-FUS+αCD40 (n=12): p = <0.0001; T-FUS (n=7) vs T-FUS+αCD40: p =0.0843; αCD40 (n=7) vs T-FUS+αCD40: p = 0.0517). (C) Focused view of tumor outgrowth at selected timepoints (day 42-53) highlighting differences between T-FUS+αCD40 non-CRs and CRs, versus αCD40 monotherapy. (D) Waterfall plot depicting CRs in the combination treatment group. (E-H) Absolute number per gram tumor and percentage of CD8 + and CD4 + T cells in the tumor and (I-L) spleen. M) tSNE plot on CD45 + cells in the TDLN. (N-Q) Absolute number and percentage of CD8 + and CD4 + T cells in the TDLN. (R) Ratio between CD8 + and CD4 + T cells in the TDLN. Significance assessed using a Welch ANOVA or Welch’s T test. *p < 0.05, **p < 0.01 vs. T-FUS+αCD40 CRs.

    Article Snippet: Following, cells were fixed with anti-mouse CD16/32 (ThermoFisher #14-0161-86) to block Fc gamma receptors for 15 mins at 4oC, then centrifuged and washed twice with 1X Flow Cytometry Staining Buffer (FACS; R&D Systems #FC001).

    Techniques: Flow Cytometry, Comparison